3D-FARE: functional 3D architectures for electrochemiluminescence applications

PRIN [2019-2022]

Abstract

The aim of 3D-FARE is to demonstrate a proof of principle for a novel platform for the highly sensitive detection of cancer cells and biomarkers. The system merges complementary expertises, where ultrabright Ir luminophores are associated to the 3D-FARE electrodes containing open porous structures and concur to the generation of intense electrogenerated chemiluminescence produced according to

the coreactant approach where the excited state is generated through the reaction between the fluorophore and the co-reactant, both oxidised at the electrode. Scientifically, the proposed strategy rests on three pillars: (i) tailored design of 3D mesoporous electrodes suited for the interaction with cells and biomolecules and their fabrication according to either top-down or bottom-up strategies; (ii) development of novel ultrabright Ir organometallic luminophores suited for the bioconjugation; (iii) assembly of an analytical platform for highly sensitive ECL imaging of cells and detection of biomarkers. The expansion and meso-structuration of the space between the biological material and the electrode surface will lead to a strong enhancement of the ECL signal finally resulting in highly sensitive new diagnostic tools for single cell analysis and cancer diagnostics.


Durata del progetto

2019-2022


Unibo Team Leader

Prof Francesco Paolucci


Responsabile Nazionale

Prof Francesco Paolucci

Partnership

Alma Mater Studiorum - Università di Bologna (Italy)
Consiglio Nazionale delle Ricerche (SCITEC)

University of Catania

Finanziamento

euro 518.059,1